Lot’s of great questions following my workshop on biomechanics and metabolic implications of obesity and OA at the 2018 annual meeting in Liverpool. Please contact me if you have any questions about the presentation.
It’s a great honor to be this year’s Medical Honoree for the Arthritis Foundation OKC Arthritis Walk. The Arthritis Foundation is the primary national organization to support arthritis patients and research. I’ve been very fortunate to received grants from the Arthritis Foundation over the years. I invite you to join our team by making a donation or walking with us on June 2nd. Help us in our goal to create a world free from joint pain so that everyone can live an active life without limitation.
More congratulations are in order for Albert and Shouan. They both earned $800 scholarly travel awards for their presentations at the Symposium: Physical Stresses- Keeping Us Alive and Well at the College of Allied Health on the OUHSC campus. Albert was recognized for his oral presentation entitled, “Dietary fat alters exercise effects on the knee: Network-based whole transcriptome analysis of mouse cartilage and subchondral bone” and Shouan for his poster presentation entitled, “Role of mitochondrial SIRT3 in chondrocyte metabolism and progenitor cell function for endogenous cartilage repair.”
Chondrocytes are mechanosensitve cells that respond to changes in the environment to maintain articular cartilage homeostasis. One potential mechanosensitive feedback system involves enzymes and signaling mechanisms that balance the reducing and oxidizing (i.e., “redox”) conditions of the cell.
We found that cyclic compressive loading of healthy bovine cartilage alters the endogenous cartilage antioxidant network. Higher compressive stress (40% strain) increased glutathione oxidation and caused a reduction in the expression of the rate-limiting enzymes for glutathione synthesis. Higher stress also increased the expression of the mitochondrial antioxidant SOD2 while decreasing the cytosolic isoform SOD1, consistent with mitochondrial-derived ROS production at higher loads. Moderate compressive stress (15% strain) caused glutathione oxidation as well, but it also increased total glutathione content and upregulated SOD1 gene expression. Pro-oxidant conditioning of cartilage using tBHP increased glutathione content without altering any other loading-associated changes. These findings suggest that glutathione synthesis, more than antioxidant enzyme upregulation, is a central pro-oxidant and mechanosensitive mediator of antioxidant defense in chondrocytes.
We lost two lab members this summer but for great reasons.
Elise accepted a faculty position at the Oregon Institute of Technology where she will be an Assistant Professor in the Department of Natural Sciences (Biology-Health Sciences Program). Elise started her new position in September.
Aaron left the lab in August to pursue his PhD in Chemical and Biological Engineering at the University of Wisconsin.
Elise and Aaron made a great team, and they will be missed. Good luck to both of them!
We were excited to host Dr. Chaofan Zhang from the University of Hong Kong in our lab for a week in May. Chaofan visited us after attending the OARSI meeting in Las Vegas, where he was honored with a plenary presentation as one of the nine highest rated abstracts by a Young Investigator. Dr. Zhang wanted to share the following comments on his visit: “I was honored to have an opportunity to visit Dr. Griffin’s lab from May.1-5. During this week, I visited their lab facilities, attended several seminars, and had deep discussions with the whole team and other scientists from the Aging & Metabolism program. It was such a fruitful trip. Everyone there was helpful to me. I am really thankful to Dr. Griffin for providing this opportunity. His exhaustive knowledge, patience and kindness always motivated me.” Thank you for the kind comments!
The Humphries and Griffin Labs are excited to have Dr. Albert Batushansky join the Aging and Metabolism Program to lead our metabolomics research. Albert received his PhD in Life Science from The Jacob Blaustein Institutes for Desert Research at the Ben-Gurion University in Negev, Israel. This is where he developed his expertise in plant metabolomics and “-omics” data integration and network analysis. He joined us most recently from Dr. Angelovici’s lab at the University of Missouri so he can pursue his passion in biomedical metabolomics research at OMRF. Welcome Albert!
Elise Donovan and Erika Barboza Lopes each had their abstracts selected for podium presentations at the 2017 Annual Meeting of the Osteoarthritis Research Society International (OARSI) this April in Las Vegas, NV. The title of Elise’s presentation is “HIGH FAT AND HIGH SUCROSE DIETS IN MICE HAVE INDEPENDENT EFFECTS ON OSTEOARTHRITIS PATHOLOGY AND CHONDROCYTE METABOLIC STRESS.” Erika’s title is “EXERCISE INDUCES TRANSIENT INFLAMMATORY AND PROFIBROTIC REMODELING OF THE INFRAPATELLAR FAT PAD.” Congratulations Elise & Erika!!